Preserved collection of dry and wet vertebrate pests

Preserved collection of dry and wet vertebrate pests  

The first part: Rat
By:
Irawan Erwin PermanaTechnical staff of BPTP Pontianak

Rats collection speciment

Collection of vertebarata pests require different treatments and ways when compared with the manufacture of collections of insect pests. With all the literature that discuss the collection of insect pests, how to make it relatively easy because it is very detailed. As for making collections of vertebrate pests is still very limited sources of information, especially in cyberspace. Therefore, I hope what has been done in BPTP Pontianak can give a little contribution in Indonesian plantation crop protection.

With all the resources that we have, we try to do the collection of vertebrate pests as one of the demands in the areas of our work as a field laboratory staff. The first thing we do is collecting insects from the field. Rats that we use comes from Segedong, one of the central areas of coconut plants in Pontianak regency.

Firstable, we killed rats with high doses of chloroform. Then we conduct screening to determine the type of collection we will do next. Screening is one of the decisions for us to make a collection of dry and wet. We decided to do a dry collection of a large rat among rats, whereas a rather small size made in the form of wet collections.

Screening of rats

The next step, we let the rats for at least 1-3 hours in a state of death so that the blood clot and does not splatter when our surgery. Perform preparation tools and materials needed to perform the surgery and skinning rats. 


Tool - a tool used namely, the base where the surgical do, rubber gloves (optional because sometimes makes working hand gloves hampered unless sensitive rubber gloves for medical standards); 10 ml syringes, scalpels and stem; sewing needle and cotton, elastic wire for a replacement the rat bones; wire cutters, collection box made of glass, styrofoam and knife. Materials - materials used, the powder to avoid fur flying rats and minimize odor, formaldehyde, cotton and camphor.

Tools and materials

Starting surgery and skinning:

1. Start cutting the neck skin of rat, do it by pinching the skin in the neck. Try not to involve the flesh of rat. Then cut crosswise ± 1cm.
2. Continue to cut the skin lengthwise toward sex mice (done with care, do not to cut in the flesh).
3. Skinning process begins by pulling the skin so that apart from flesh (such as pulling clothes from the body) with care so will not to tear the skin or flesh come.
4. Skin pulled up to a foot - the foot of a mouse in his wrist.
5. Cut the foot of the rats in the wrist, prepare tissue for the anticipated release of blood.6. Then massage the rat tail, gently pull the tail bone out of his skin sheath.
6. The last part of the process of skinning is to cut the neck of rats, so the only remaining part of the head and legs - legs of rats.After the skinning process is complete then the next part is to prepare the ingredients - ingredients for the filling mouse skin with cotton and then stitched. Prepare the syringe, then suck up to the limit of formaldehyde in 5 ml bottles. Insert the thread into the needle, with a length of yarn for sewing adjusted estimates along the body of rats from start sexes up to the neck of rats.

to be continued with pics...